A Rapid and Selective method for the Quantification of Naringenin in order to monitor Naringinase activity
نویسندگان
چکیده
Sodium acetate (NaOAc) can selectively ionize 7-OH of naringenin generating a bathochromic shift of 41nm in its UV absorbance. This principle is used in the described spectrophotometric method that can detect naringenin amidst naringin and prunin present in the incubation mixture of naringinase. The method could be adopted for real sample analysis as it remains unaffected by the presence of phenolics such as ascorbic acid, gallic acid, citric acid and cinnamic acid.
منابع مشابه
Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective α-L-Rhamnosidase and β-D-Glucosidase Activities of Naringinase
The production of flavonoid glycosides by removing rhamnose from rutinosides can be accomplished through enzymatic catalysis. Naringinase is an enzyme complex, expressing both α-L-rhamnosidase and β-D-glucosidase activities, with application in glycosides hydrolysis. To produce monoglycosylated flavonoids with naringinase, the expression of β-D-glucosidase activity is not desirable leading to t...
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